Madonna has appeared to come out as gay while taking part in a TikTok challenge.The pop icon, 64, was seen in a bathroom trying to toss a pair of hot pink knickers into a bin but missing.Madonna wrote alongside the post: "If I miss, I’m gay!"The mum-of-six wrote then dramatically turned around, as she threw her hand up in the air.Fans were left puzzled by the video and took to the comments section to debate whether Madonna was making a revelation about her sexuality.One asked: "Coming out at 64?"READ MORE: Madonna baffles fans as she eats and sips wine in fully clothes bath snapsAnother added: "Did I just witness Madonna coming out, good for her."Madonna has only ever publicly dated men, including Tupac Shakur, and Vanilla Ice.Madonna has also been married twice, to Sean Penn between 1985 and 1989 and to Hollywood producer Guy Ritchie, between 2000 and 2008.She also dated dancer Carlos Leon, who is the father of her daughter Lourdes, 25.While she has never dated a woman she famously locked lips with Britney Spears and Christina Aguilera at the 2003 MTV Video Music Awards.Madonna also shared a snap on social media, showing her and Britney recreating their kiss at the Toxic hitmaker's surprise wedding to Sam Asghari back in June.She has also been seen grinding against Dominican rapper Tokischa for a remix of one of her songs, and kissing her snaps she shared on social media.Madge also shared snaps of herself smooching with two women in the back of a car while celebrating her 64th birthday in Italy.In 2008 Madonna kissed one of her female backing dancers during a special private show in Paris.She said afterwards: "Why do I have this relationship with France? ‘I’m always drawn to working with French people – and frenching.